ORG.one is a pilot-stage project designed to support faster, more localised sequencing of critically endangered species, by enabling biologists to rapidly sequence those species close to the sample’s origin, using the latest ultra-long read approaches.
Current projects to sequence endangered species have made progress, however they can be limited by complex workflows and the requirement to send samples to centralised, often overseas, locations for sequencing.
Data-rich, de novo whole genome assemblies will be enabled through the provision of consumable support that can be used with Oxford Nanopore sequencers, on the condition that the data generated will be openly shared with the scientific community.
- ORG.one is supported by Oxford Nanopore, the maker of scalable, real-time sequencing devices.
- The pilot phase of this project will support Oxford Nanopore’s current user Community to develop high quality de novo assemblies of genomes of a number of species in the IUCN Red list.
- A small number of users who currently have access to these samples will be supported during the pilot phase.
- These users will receive free-of-charge consumables, to enable them to sequence species from the IUCN Red List, with the latest nanopore tools and methods.
- These users will be supported in the generation of long read libraries from their samples, to aim for the most comprehensive genome possible.
- Free-of-charge consumables will be provided on the condition that the data will be uploaded to the EBI public database within 6 weeks of receiving them.
- The aim is to gather feedback during the pilot phase so that we can consider the best way to expand ORG.one to a broader community in the future.
- Researchers must be responsible for any local legal and ethical permits to generate the data and to share or export it. Finally, we stipulate that no animals are harmed or killed for their DNA.
- Existing nanopore users can register their interest to take part in the pilot.